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目的探讨在失事潜艇固壳未破损的环境下机体淋巴细胞分类计数改变情况,从而进一步探讨机体免疫功能的变化。方法4名健康男性受试者,利用500 m饱和潜水系统模拟失事潜艇固壳未破损的环境条件,控制氧浓度(18±1)％,二氧化碳浓度(2±0．5)％,气温10～18℃,应急照明,应急营养供给。分别于试验前,暴露第2天、第4天、第8天早晨空腹抽血,流式细胞分析术检测淋巴细胞分类。结果在试验开始后T细胞百分比升高,但随后逐渐下降,趋近正常值;B细胞百分比下降,在第8天有显著降低;NK细胞在暴露第2天显著下降,随后有恢复趋势;Tc下降,与试验前相比有显著差异; Th升高,但不显著。结论失事潜艇固壳未破损的环境条件对人体血液淋巴细胞分类有一定的影响,机体免疫细胞分类计数变化提示免疫功能下降。
Objective To investigate the change of classification and counting of lymphocytes in unbreakable solid shell of a crash submarine so as to further explore the changes of immune function. Methods Four healthy male subjects were used to simulate the unbreakable solid shell environment of the failed submarine with a 500 m saturated diving system to control the oxygen concentration (18 ± 1)%, the concentration of carbon dioxide (2 ± 0.5)%, the temperature of 10 ~ 18 ℃, emergency lighting, emergency nutrition supply. Fasting blood samples were collected on day 2, day 4 and day 8 respectively before the test, and the lymphocyte categories were detected by flow cytometry. Results The percentage of T cells increased at the beginning of the experiment, but then gradually decreased and approached the normal value. The percentage of B cells decreased and decreased significantly on the 8th day. The NK cells decreased significantly on the 2nd day of exposure and then recovered. Tc Decreased, compared with before the test were significantly different; Th increased, but not significant. Conclusion The unbreakable environmental conditions of the solid shell of the crash submarine have a certain impact on the classification of human blood lymphocytes. The change of the classification and counting of immune cells in the population indicates that the immune function is decreased.