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目的检测青藏铁路沿线鼠疫菌是否具有公认的毒力因子。方法采用反向间接血凝试验检测FraI;利用假结核菌对PstI敏感可产生抑菌环的复盖种子层法检测PstI;采用乏Ca2+时37℃鼠疫菌不能生长观察Vwa,利用色素培养基观察Pgm。结果所检测青藏铁路沿线菌株都具有PstI+、Vwa+、FraI+、Pgnn+4种毒力因子。结论根据其地理位置和宿主判断所检测菌株属于强毒力菌株。
Objective To test whether there is a recognized virulence factor of Y. pestis along the Qinghai-Tibet Railway. Methods The reverse indirect hemagglutination test was used to detect FraI. Pseudomonas fluorescens PstI was sensitive to PstI to produce an antibacterial ring covered with seed layer method to detect PstI. When Ca 2+ deficiency was used, 37 ℃ plague bacteria could not grow to observe Vwa, Pgm. Results The strains tested along the Qinghai-Tibet Railway had four virulence factors, PstI +, Vwa +, FraI + and Pgnn +. Conclusion According to their geographical location and host judgment, the tested strains belonged to virulent strains.