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对苹果砧术试管苗继代培养过程中形成玻璃苗的可能因素，如瓶内湿度、琼脂浓度、细胞分裂素浓度进行比较试验，观察玻璃苗的形成及变化情况．结果表明：瓶内湿度过大，琼脂浓度略低，细胞分裂素浓度过高，都易引起玻璃化现象，而改变这些因素，可以有效地预防和缓解玻璃苗的发生．
The possible factors of forming glass seedlings in the subculture process of apple rootstocks, such as the humidity in the bottle, the concentration of agar and the concentration of cytokinin were compared to observe the formation and changes of the glass seedlings. The results showed that: excessive humidity in the bottle, slightly lower concentration of agar, high concentrations of cytokinin are likely to cause vitrification, and changing these factors can effectively prevent and alleviate the occurrence of glass seedlings.