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【目的】建立布鲁氏菌的16S rDNA序列分析方法,评价该方法鉴定布鲁氏菌的特异性和实用性。【方法】用PCR扩增布鲁氏菌的16S rDNA片段,将扩增的产物纯化后测序,从GenBank下载与布鲁氏菌易发生血清学交叉反应的细菌的16S rDNA序列。使用DNAMAN软件进16S rDNA序列相似性分析。【结果】在布鲁氏菌中16S rDNA核苷酸序列相似性达到了99.74%,而与其他有血清型交叉反应的菌株相比较,16S rDNA序列间有显著差异。【结论】16S rDNA序列分析是一种快速、简便、特异的鉴定布鲁氏菌的方法之一。
【Objective】 To establish a 16S rDNA sequence analysis method of Brucella and evaluate the specificity and practicability of this method in identifying Brucella. 【Method】 The 16S rDNA fragment of Brucella was amplified by PCR. The amplified product was purified and sequenced. The 16S rDNA sequence of the bacteria that was serologically cross-reactive with Brucella was downloaded from GenBank. Similarity analysis of 16S rDNA sequences using DNAMAN software. 【Result】 The results showed that the similarity of 16S rDNA sequence in Brucella was 99.74%, but there was a significant difference between 16S rDNA sequence and other serotype cross-reacting strains. 【Conclusion】 16S rDNA sequence analysis is a rapid, simple and specific method for the identification of Brucella.