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目的 探讨GPA基因突变频率检测作为累积受照生物剂量计的可行性。方法 用改进的GPA基因位点突变检测技术 ,分析了 5 5名不同时期开始X射线工作者和 5 0名非放射科医务工作者外周血红细胞GPA基因突变变异体频率与开始工作时期、工龄和累积受照剂量间的关系。结果 医用X射线工作者的GPA基因突变频率明显高于对照 ;突变率明显增高是发生在 1970年前开始X射线工作者 ,这与肿瘤流行病学调查所见恶性肿瘤危险明显增高发生在 1970年前开始X射线工作者相一致 ;X射线工作者的GPA基因突变频率随累积剂量而增高 ,剂量 效应关系N突变优于NN突变。结论 用GPA基因突变检测作为生物剂量计 ,还有许多问题需要解决 ,如 :个体差异、特异性及剂量 效应刻度曲线等
Objective To investigate the feasibility of detecting the mutation frequency of GPA gene as a cumulative biological dosimeter. Methods The mutation frequency of GPA gene in peripheral blood erythrocytes of 55 initiating X-ray workers and 50 non-radiological medical workers in 55 different periods was compared with that of the beginning working period, length of service and Cumulative dose relationship. Results The frequency of GPA mutation in medical X-ray workers was significantly higher than that in controls; the significantly higher mutation rate occurred in X-ray workers before 1970, which was associated with a marked increase in the risk of malignant tumors seen in the epidemiological survey of tumors occurred in 1970 X-ray workers with X-ray workers consistent with the same frequency; X-ray workers GPA gene mutation frequency increases with the cumulative dose, dose-response N mutation better than the NN mutation. Conclusion There are many problems to be solved by using GPA mutation detection as biological dosimeters, such as individual difference, specificity and dose-response curve