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选取 ( 4～ 6)周龄雄性 Balb/ C小鼠 ,病毒感染组腹腔接种不同浓度的 CVB3病毒液 ,对照组注射 RPMI1 640液 ,7d后测定血清中和抗体效价及乳酸脱氢酶 ( LDH)活性 ;测定心肌组织的病毒滴度及观察心肌组织病理学改变。结果显示 :( 1 )病毒感染组鼠血清中和抗体效价及乳酸脱氢酶活性显著高于正常对照组 ,且随接种 CVB3浓度增加 ,L DH活性增高。( 2 )随接种 CVB3浓度增高 ,小鼠心肌组织病毒滴度增加不明显 ;( 3)病毒感染组心肌组织出现不同程度的灶性病理改变。 ( 4 )接种病毒浓度与鼠心肌炎发病率之间不存在剂量反应关系。
The male Balb / C mice (4-6 weeks old) were selected and inoculated intraperitoneally with different concentrations of CVB3 virus solution. The control group was injected with 640 μl of RPMI140 solution. After 7 days, serum neutralizing antibody titer and lactate dehydrogenase ) Activity was measured; the titer of myocardium was measured and pathological changes of myocardium were observed. The results showed that: (1) The serum neutralizing antibody titer and lactate dehydrogenase activity in virus-infected mice group were significantly higher than those in normal control group, and the activity of L DH increased with the CVB3 concentration inoculated. (2) With the increase of CVB3 inoculation, the increase of virus titer in myocardium of mice was not obvious; (3) Focal pathological changes of myocardium were observed in virus infected group. (4) there is no dose-response relationship between inoculation virus concentration and the incidence of myocarditis.