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目的:观察消痰散结方对胃癌组织增殖细胞核抗原(PCNA)表达的影响,探讨消痰散结方抑制胃癌细胞增殖的作用机制。方法:建立裸鼠人胃癌MKN-45原位移植模型,采用随机数字表法随机分成生理盐水组,消痰散结方低、中、高剂量组,5-氟尿嘧啶(5-Fu)组,每组8只。观察指标有:①观察各组荷瘤鼠肿瘤生长情况及抑瘤率;②观察消痰散结方干预胃癌组织PCNA蛋白表达的影响。结果:消痰散结方对裸鼠人胃癌原位移植瘤有较好的抑制肿瘤生长作用,消痰散结方低、中、高剂量组抑瘤率分别为36.62%、44.82%、58.11%,呈现出剂量依赖效应。结论:消痰散结方有较好的抑制肿瘤生长的作用,其抑瘤作用机制可能与下调肿瘤细胞PCNA蛋白的表达有关,进而抑制细胞增殖、分裂,控制胃癌的复发与转移。
Objective: To observe the effect of Xiaotan Sanjie Fang on the expression of proliferating cell nuclear antigen (PCNA) in gastric cancer tissues, and to explore the mechanism of Xiaozhi Sanjie recipe in inhibiting the proliferation of gastric cancer cells. METHODS: A model of orthotopic MKN-45 gastric cancer transplanted in nude mice was established. Randomly divided into normal saline group and low-, medium-, and high-dose groups of Xiaozao Sanjie group, 5-fluorouracil (5-Fu) group by random number table method. Group 8 only. Observed indicators are: 1 observe the tumor growth and tumor inhibition rate of tumor-bearing mice in each group; 2 observe the effect of Xiaotan Sanjie Prescription on the expression of PCNA protein in gastric cancer tissue. RESULTS: Xiaotan Sanjie Recipe inhibited the growth of human gastric cancer orthotopic xenograft tumor in nude mice. The tumor inhibition rate of Xiaozhan Sanjie Fang was 36.62%, 44.82%, 58.11%, respectively. , showing a dose-dependent effect. Conclusion: Xiaotan Sanjie Recipe has a good effect on inhibiting tumor growth. Its anti-tumor mechanism may be related to the down-regulation of PCNA protein expression in tumor cells, thereby inhibiting cell proliferation and division, and controlling the recurrence and metastasis of gastric cancer.