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目的了解海南省发热病人中感染恙虫病东方体的状况,并确定其基因分型。方法利用巢氏PCR方法,检测海南省各家医院2009~2010年送检的117份不明原因发热病人血标本的groEL基因的特异性片段,对所检测到的部分目的片段进行基因测序,从基因水平进一步证实阳性结果,并对序列同源性及进化分析。结果从117份发热病人血标本中发现有恙虫病东方体阳性15份,检出率为12.8%。取10份PCR阳性产物进行测序,核酸序列基本一致,彼此间仅存在3个核苷酸的差异,构建进化树进行聚类分析,结果示海南省恙虫病东方体序列与R.tsutsugamushi(M31887)恙虫病东方体在同一分支上,同源性高。结论海南省发热病人中存在恙虫病东方体感染的状况,应加强相关监测和防控措施,为给海南省制定恙虫病防治策略提供科学依据。
Objective To understand the status of oriental tsutsugamushi infection in patients with fever in Hainan Province and to determine the genotypes. Methods The nested PCR method was used to detect the specific fragments of groEL gene in 117 blood samples from patients with unexplained fever in different hospitals in Hainan Province from 2009 to 2010. The partial fragments of groEL gene were sequenced. The level further confirms the positive result, and sequence homology and evolutionary analysis. Results From 117 blood samples of febrile patients, there were 15 positive orientalis tsutsugamushi, the detection rate was 12.8%. Ten PCR positive products were sequenced and their nucleic acid sequences were basically the same. There were only three nucleotide differences between them. The phylogenetic tree was constructed for clustering analysis. The results showed that the ORF was consistent with R. tsutsugamushi (M31887) Orientia tsutsugamushi body in the same branch, high homology. Conclusion There is an infection status of Oriental body with tsutsugamushi disease in febrile patients in Hainan Province. Therefore, relevant monitoring and control measures should be strengthened to provide a scientific basis for the prevention and control strategy of tsutsugamushi in Hainan Province.