论文部分内容阅读
目的：研究血管内皮细胞生长因子（ＶＥＧＦ）在儿童恶性胶质瘤的表达及其与血管生成，细胞增殖的关系。方法：应用免疫组化方法检测３３例儿童恶性胶质瘤组织中ＶＥＧＦ表达与微血管数（ＭＶＱ）、ＰＣＮＡ标记指数（ＰＣＮＡＬＩ）。结果：３３例儿童恶性胶质瘤ＶＥＧＦ表达阳性２３例（６９．７％）；ＶＥＧＦ表达阳性肿瘤ＭＶＱ、ＰＣＮＡＬＩ显著高于ＶＥＧＦ表达阴性肿瘤（Ｐ＜０．００５）；肿瘤组织ＶＥＧＦ表达与ＭＶＱ、ＰＣＮＡＬＩ显著相关（相关系数ｒ分别为０．５２和０．３７，Ｐ＜０．００１）。结论：儿童恶性胶质瘤细胞可以合成ＶＥＧＦ，ＶＥＧＦ在肿瘤血管生成及细胞增殖中起重要作用。
Objective: To study the expression of vascular endothelial growth factor (VEGF) in glioblastoma in children and its relationship with angiogenesis and cell proliferation. Methods: Immunohistochemistry was used to detect the expression of VEGF, microvessel count (MVQ) and PCNA labeling index (PCNALI) in 33 cases of malignant glioma. Results: The expression of VEGF was positive in 23 cases (69.7%) of malignant gliomas in 33 children; the MVQ and PCNALI of VEGF expression positive tumors were significantly higher than those of VEGF expression negative tumors (P<0.005); the expression of VEGF and MVQ in tumor tissues. PCNALI was significantly associated (correlation coefficients r were 0.52 and 0.37, respectively, P<0.001). Conclusion: The glioma cells in children can synthesize VEGF and VEGF plays an important role in tumor angiogenesis and cell proliferation.