论文部分内容阅读
本研究以柑桔优良品种不知火(shinanui)为试料,通过携带质粒pBI121-A的根癌农杆菌转化成年树腋芽,获得转甲型肝炎病毒(HAV)衣壳蛋白融合基因不知火植株。研究结果表明,锦橙试管砧木培养15d时不知火成年树腋芽的嫁接成活率最高,达到90%;不知火不同成熟度的枝条应采用不同的消毒时间,木质化的枝条消毒时间要长些,半木质化的枝条消毒时间要短些。对获得的拟转化植株进行PCR分析以及对PCR阳性植株进行测序,结果表明HAV衣壳蛋白融合基因已整合到不知火的基因组中。
In this study, shinanui, an elite citrus cultivar, was used as a test sample and Agrobacterium tumefaciens strain carrying plasmid pBI121-A was transformed into axillary buds of adult trees. The results showed that the grafted survival rate of juvenile tree axillary buds was the highest at 90 days after being cultured for 15 days in the medium of Jincheng, and the disinfection time was different for the branches with different maturity of fire. The disinfection time of lignified branches was longer, The shorter branches of disinfection time. PCR analysis of the resulting plants to be transformed and sequencing of PCR-positive plants revealed that the HAV capsid fusion gene has been integrated into the unaware genome.