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以７４１杨和苹果矮化砧Ｍ２６和Ｍ７为材料，研究了影响７４１杨试管苗玻璃化现象的因素。除培养基中ＢＡ和ＩＢＡ的浓度、琼脂浓度外，光强和温度组合是影响试管苗玻璃化现象的重要因素。７４１杨试管苗玻璃化与其内源ＡＢＡ水平有关。７４１杨无根试管苗的玻璃化率和器官分化能力存在季节变化，春季和夏季较强，冬季较弱；７４１杨无根试管苗的内源ＡＢＡ含量则是春季和夏季低，冬季高。无根正常试管苗ＡＢＡ含量为无根玻璃苗的５．５９倍。苹果无根玻璃化试管苗如在其培养基中加入低浓度（Ｏ．１～０．５ｍｇ／Ｌ）ＡＢＡ，或在较强的光照条件下培养，可以转化为无根正常试管苗，并能正常进行继代和生根。
The effects of 741 poplar and apple dwarfing anvil M26 and M7 on the vitrification of 741 young plantlets were studied. In addition to the medium concentration of BA and IBA, agar concentration, the combination of light intensity and temperature is an important factor affecting the vitrification of plantlets. 741 Young plantlet vitrification is related to its endogenous ABA level. 741 There was a seasonal variation in the vitrification rate and organ differentiation ability of test-tube plantlets, which was stronger in spring and summer and weaker in winter. The endogenous ABA content of test-tube plantlets of A. przewalskii 741 was lower in spring and summer and higher in winter. The ABA content of rootless test-tube seedlings was 5.59 times of that of non-rooted ones. Apple rootless glassy plantlets such as in their medium by adding a low concentration (O. 1 ~ 0.5mg / L) ABA, or under strong light conditions can be transformed into non-rooted normal plantlets, and can Normal subculture and rooting.