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本研究的目的是克隆广西巴马小型猪SLC30-A8基因,并确定各组织的表达量。利用RT-PCR的方法扩增并克隆SLC30-A8基因;荧光定量PCR检测SLC30-A8基因在12月龄广西巴马小型猪心脏、肝脏、脾脏、肺脏、肾脏、胰腺、小肠和脂肪中的表达。结果显示,成功克隆广西巴马小型猪SLC30-A8基因CDS区全长1 110 bp,荧光定量PCR结果显示SLC30-A8基因在12月龄广西巴马小型猪胰腺组织中表达量最高,其次是心脏、脾脏,在肺脏、小肠以及脂肪组织中几乎不表达。本研究成功克隆了广西巴马小型猪SLC30-A8基因并确定在胰腺组织中表达最高。该研究将为下一步研究SLC30-A8基因在糖尿病的发生过程中对糖代谢的作用奠定基础。
The purpose of this study was to clone the SLC30-A8 gene of Guangxi Bama miniature pig and to determine the expression level in each tissue. The gene SLC30-A8 was amplified and cloned by RT-PCR. The expression of SLC30-A8 gene in heart, liver, spleen, lung, kidney, pancreas, small intestine and adipose tissue of 12-month- . The results showed that the CDS region of SLC30-A8 gene in Guangxi Bama miniature pig was successfully cloned, which was 1 110 bp in length. Fluorescence quantitative PCR results showed that the expression level of SLC30-A8 gene was the highest in pancreatic tissues of Bama miniature pigs at 12 months of age, , Spleen, almost no expression in lung, small intestine and adipose tissue. This study successfully cloned Guangxi Bama miniature pig SLC30-A8 gene and to determine the highest expression in the pancreas. This study will lay the foundation for further studies on the role of SLC30-A8 in glucose metabolism during the development of diabetes mellitus.