论文部分内容阅读
Objective To investigate the variability of human cytomegalovirus(HCMV)UL140 open reading frame(ORF)in clinical strains,and to explore the relationship between the variability of UL140 ORF and different symptoms of HCMV infection.Methods HCMV UL140 ORF was amplified by polymerase chain reaction and sequenced selectedly in 30 clinical strains.Results UL140 ORF of all clinical strains was amplified successfully.Compared with that of Toledo strain,the nucleotide and amino acid sequence identities among all strains were 96.5%-100.0% and 95.2%-100.0%,respectively.All of the nucleotide changes were substitutions.The post-translational modification sites were conserved.The result of phylogenetic tree showed that the strains did not cluster according to different clinical symptoms.Conclusion HCMV UL140 ORF in clinical strains is highly conserved,which may play an important role in HCMV infection.
Objective To investigate the variability of human cytomegalovirus (HCMV) UL140 open reading frame (ORF) in clinical strain, and to explore the relationship between the variability of UL140 ORF and different symptoms of HCMV infection. Methods HCMV UL140 ORF was amplified by polymerase chain reaction and sequenced selectedly in 30 clinical strains. Results UL140 ORF of all clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identities among all strains were 96.5% -100.0% and 95.2% -100.0%, respectively .All of the nucleotide changes were compared. The post-translational modification sites were conserved. The result of phylogenetic tree showed that the strain did not cluster according to different clinical symptoms. Confluence HCMV UL140 ORF in clinical disease is highly conserved, which may play an important role in HCMV infection.