The study of 3-dimensional structures of IgG with atomic force microscopy

• 作 者: 郁毅刚 徐如祥 姜晓丹 柯以铨
• 机 构: Department of Neurosurgery,Zhujiang Hospital,South Medical University,Guangzhou510282,China,Departme
• 出 处: Chinese Journal of Traumatology
• 发表日期: 2005年05期
• 关键词: IgG elliptical spherical detecting rabbit polyclonal length scanner aspartate mi

Objective: To detect 3-dimensional images of anti-N-methyl-D-aspartate receptor Nr1 (NMDAr1) polycolonal IgG affixed on mica in physiological environment. Methods: The images and data were obtained from a contact mode and commercial Si_3N_4 probed tip by using atomic force microscope (AFM). Results: The anti-NMDAr1 polycolonal IgG has a characteristic structure described as an ellipse spherical shape of 136.4 × 62.8 × 26.1 . On the section of the ellipse edge there were two peaks about 13nm in width. Conclusions: Using AFM to investigate biomacromolecule can make us deeply understand the structure of IgG, which will instruct us to detect the membrane receptor protein as a labelling agent.

Objective: To detect 3-dimensional images of anti-N-methyl-D-aspartate receptor Nr1 (NMDAr1) polycolonal IgG affixed on mica in physiological environment. Methods: The images and data were obtained from a contact mode and commercial Si_3N_4 probed tip by using atomic force microscope (AFM). Results: The anti-NMDAr1 polycolonal IgG has a characteristic structure described as an ellipse spherical shape of 136.4  × 62.8  × 26.1 . On the section of the ellipse edge there were two peaks about 13nm in width. Conclusions: Using AFM to investigate biomacromolecule can make us deeply understand the structure of IgG, which will instruct us to detect the membrane receptor protein as a labelling agent.