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目的研究盐酸多柔比星神经节介入对脑缺血再灌注模型大鼠血管内皮生长因子(VEGF)、过氧化物歧化酶(SOD)及丙二醛(MDA)水平的影响。方法30只Wistar大鼠按体重随机分为模型组、实验组以及空白组,每组各10只。用Pulsinelli四血管阻断法,建立脑缺血再灌注模型。实验组于术前48 h,开始于椎间孔缓慢注射盐酸多柔比星1 mg·kg-1,模型组与空白组注射同等剂量生理盐水q12 h。以酶联免疫吸附法(ELISA)测定大鼠脑组织内VEGF、SOD及MDA水平。结果在给药12,24 h的神经功能评分,与空白组的0分比较,模型组分别为(2.41±0.25),(1.98±0.23)分,明显增加,2组比较差异有统计学意义(P<0.05);与模型组比较,实验组分别为(1.58±0.19),(1.31±0.15)分,明显降低,2组比较差异有统计学意义(P<0.05)。模型组在给药12,24 h,脑组织VEGF表达分别为124.63±15.63,120.57±12.84,血清中丙二醛水平分别为(3.56±0.38),(3.85±0.35)mmol·mg~(-1);实验组在给药12,24 h,脑组织VEGF表达分别为102.26±12.38,100.47±11.91,血清中丙二醛水平分别为(2.97±0.35),(3.25±0.35)mmol·mg~(-1),较模型组明显降低,2组比较差异有统计学意义(P<0.05)。在给药12,24 h,模型组血清中SOD分别为(120.48±15.03),(119.37±12.58)U·mg~(-1);实验组血清中SOD水平分别为(182.73±19.49),(181.57±18.63)U·mg~(-1),实验组较模型组明显升高,2组比较差异有统计学意义(P<0.05)。结论盐酸多柔比星神经节介入通过改善氧化应激反应以及炎症反应,从而保护脑缺血再灌注大鼠脑组织缺血再灌注损伤。
Objective To investigate the effects of doxorubicine on the level of vascular endothelial growth factor (VEGF), superoxide dismutase (SOD) and malondialdehyde (MDA) in cerebral ischemia-reperfusion model rats. Methods Thirty Wistar rats were randomly divided into model group, experimental group and blank group according to body weight, with 10 rats in each group. Pulsinelli four-vessel occlusion method was used to establish cerebral ischemia-reperfusion model. The experimental group began to slowly injected intraperitoneal injection of doxorubicin 1 mg · kg-1 48 h before the model group and the blank group received the same dose of saline q 12 h. The levels of VEGF, SOD and MDA in rat brain were determined by enzyme-linked immunosorbent assay (ELISA). Results The scores of neurological function at 12 and 24 h after drug administration were (2.41 ± 0.25) and (1.98 ± 0.23) points higher than those in the blank group (P <0.05), and the difference was statistically significant P <0.05). Compared with the model group, the experimental groups were (1.58 ± 0.19) and (1.31 ± 0.15) points, respectively, which were significantly lower than those in the model group. There was significant difference between the two groups (P <0.05). The expression of VEGF in brain tissue of model group was 124.63 ± 15.63 and 120.57 ± 12.84 respectively at 12 and 24 h after administration, the levels of MDA in serum were (3.56 ± 0.38) and (3.85 ± 0.35) mmol · mg ~ (-1) ). The expression of VEGF in the experimental group was (102.26 ± 12.38), (100.47 ± 11.91) and (2.97 ± 0.35), (3.25 ± 0.35) mmol · mg ~ -1), which was significantly lower than the model group, the difference between the two groups was statistically significant (P <0.05). The levels of SOD in serum of model group were (120.48 ± 15.03) and (119.37 ± 12.58) U · mg -1 at 12 and 24 h, respectively. The levels of SOD in the experimental group were (182.73 ± 19.49) and 181.57 ± 18.63) U · mg ~ (-1), the experimental group was significantly higher than the model group, the difference between the two groups was statistically significant (P <0.05). Conclusion Doxorubicin hydrochloride ganglioside can protect brain against ischemia-reperfusion injury by improving oxidative stress reaction and inflammatory reaction.