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目的:以不同浓度的聚氧乙烯脱水山梨醇单油酸酯(吐温-80)为对象,建立一种简便易行的清醒小动物类过敏试验方法,用于中药注射剂的类过敏反应筛选和评价。方法:所有受试物均含有0.4%伊文思蓝,各组分别iv给予受试药小鼠20 mL.kg-1,豚鼠30 mL.kg-1,给药后观察30 min内动物的行为学变化,耳廓蓝染情况,测定小鼠双耳丙酮生理盐水浸液的吸光度。结果:小鼠iv 0.5%～10%吐温-80溶液、含0.5%～10%吐温-80的鱼腥草蒸馏液、150～600 mL.kg-1双黄连粉针剂;豚鼠iv 0.25%～1.0%吐温-80溶液、含0.25%～1.0%吐温-80的鱼腥草蒸馏液、54 mg.kg-1双黄连粉针剂,均可造成小鼠和豚鼠耳廓不同程度蓝染,并且各组随着剂量增高,耳廓蓝染面积增大、染色加深。鼠耳经丙酮生理盐水液浸泡后,用半自动生化仪检测,发现伊文思蓝渗出量与肉眼观察结果基本一致。结论:利用小鼠和豚鼠耳廓血管通透性测试,可以作为筛选中药注射剂类过敏反应的模型,是一种简便易行的清醒小动物类过敏试验方法。
OBJECTIVE: To establish a simple and easy method for detecting allergic small animal allergy using different concentrations of polyoxyethylene sorbitan monooleate (Tween-80) Evaluation. Methods: All the tested samples contained 0.4% Evans blue. The mice in each group were treated with 20 mL.kg-1 of test drug and 30 mL.kg-1 of guinea pig respectively. The animals were observed behaviorally Changes in the auricle blue staining, determination of mouse ears acetone acetone solution absorbance. Results: Mice iv 0.5% ~ 10% Tween-80 solution, Houttuynia cordata extract containing 0.5% ~ 10% Tween-80, Shuanghuanglian powder 150 ~ 600ml.kg- ~ 1.0% tween -80 solution, containing 0.25% ~ 1.0% Tween -80 Houttuynia distillate, 54 mg.kg-1 Shuanghuanglian injection, can cause different degrees of blue-pigmented mouse and guinea pig ear , And each group with the dose increased, blue ear area increased, staining deepened. Mouse auricular saline immersed in saline, semi-automatic biochemical analyzer test and found Evans blue ooze and the naked eye observation results are basically the same. Conclusion: The use of mouse and guinea pig auricle vascular permeability test can be used as a screening model of traditional Chinese medicine injection allergic reaction is a simple and easy method of awake small animal allergy test.