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作者研究了对锑剂有高度抗药性的利什曼原虫突变体对两种二线药物的敏感性。婴儿利什曼原虫无鞭毛体置25 cm2烧瓶内用无细胞的MAA/20培养基,在5％CO2,37℃条件下作纯培养。每周传代一次。克隆化的野生型原虫已适应于0.5μg/ml酒石酸锑钾
The authors investigated the susceptibility of two Leishmania mutants to two second-line drugs that are highly resistant to antimony agents. Leishmania infantum flagella was placed in 25 cm2 flasks with cell free MAA / 20 medium, 5% CO2, 37 ℃ under the conditions of pure culture. Passage once a week. The cloned wild type protozoa have been adapted to 0.5 μg / ml antimony potassium tartrate