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目的探讨匹罗卡品诱导癫痫模型的内源性神经干细胞的增殖、迁移,从而研究癫痫发生后的神经发生及可塑性改变。方法氯化锂和匹罗卡品联合诱导大鼠癫痫模型,分为对照组、癫痫发作后3、7、14、28 d组,用免疫组织化学方法动态检测大鼠海马多唾液酸神经细胞粘附分子(polysiolylated neural cell ad-hesion molecule,PSA-NCAM)及PSA-NCAM/5-溴脱氧尿苷嘧啶(bromodeoxyuridine,BrdU)的表达。结果与对照组比较,海马齿状回(dentate gyrus,DG)PSA-NCAM及PSA-NCAM/BrdU的阳性细胞在癫痫后3 d开始增加(P<0.05),14 d达到高峰(P<0.05),28 d后开始下降,但仍高于对照组(P<0.05)。结论癫痫发生后出现神经干细胞的增殖、迁移,也即出现了神经发生和可塑性改变。
Objective To investigate the proliferation and migration of endogenous neural stem cells induced by pilocarpine induced epilepsy in order to study the changes of neurogenesis and plasticity after epilepsy. Methods The model of epilepsy induced by lithium chloride and pilocarpine was induced in rats. The rats were divided into control group, 3, 7, 14, and 28 d after seizures. The hippocampus polysaccharides were dynamically detected by immunohistochemistry (PSA-NCAM) and PSA-NCAM / bromodeoxyuridine (BrdU) were detected by immunohistochemistry. Results Compared with the control group, the number of positive cells in PSA-NCAM and PSA-NCAM / BrdU of dentate gyrus (DG) in hippocampus increased at 3 days (P <0.05) and reached the peak at 14 days (P <0.05) After 28 days, it began to decline, but still higher than the control group (P <0.05). Conclusion Proliferation and migration of neural stem cells occur after epilepsy, that is, neurogenesis and plasticity change occur.