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目的:观察出生后注射雌二醇诱导小鼠隐睾模型的发生率及生精情况。方法:90只雄性新生Balb/C小鼠随机分为实验组(n=60)、溶剂对照组(n=20)和正常对照组(n=10)。实验组进一步随机分为4个亚组,分别于出生后3～28 d(4周组)、3～42 d(6周组)、3～56 d(8周组)、3～70 d(10周组)给予皮下注射17-β雌二醇(5μg/d)。观察停药后2周隐睾发生率及形态学变化。结果:实验组各亚组(4、6、8、10周组)停药后2周隐睾发生率分别为0%、26.7%、60%、60%。而对照组均无隐睾发生。在4周和6周组停药后均出现隐睾自行下降恢复生精的情况,连续注药8周后模型稳定,隐睾生精不恢复。结论:出生后连续注射雌二醇8周能够建立稳定的隐睾性不育小鼠模型。
Objective: To observe the incidence and the spermatogenesis of cryptorchidism induced by estradiol in mice after birth. Methods: Ninety male Newborn Balb / C mice were randomly divided into experimental group (n = 60), solvent control group (n = 20) and normal control group (n = 10). The experimental groups were further randomly divided into 4 subgroups: 3 to 28 days after birth (4 weeks), 3 to 42 days (6 weeks), 3 to 56 days (8 weeks), 3 to 70 days 10 weeks group) were given subcutaneous injections of 17-β estradiol (5 μg / d). Observe the incidence and morphological changes of cryptorchidism two weeks after stopping. Results: The incidences of cryptorchidism were 0%, 26.7%, 60% and 60% in the 2 weeks after drug withdrawal in each subgroup (4, 6, 8 and 10 weeks) in the experimental group. The control group had no cryptorchidism. Cryptorchidism self-descended and resumed spermatogenesis after 4 weeks and 6 weeks of withdrawal. The model was stable after 8 weeks of continuous injection, and cryptorchidism did not recover. CONCLUSIONS: Continuous injection of estradiol for 8 weeks after birth can establish a stable mouse model of cryptorchidism.