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目的 :通过细胞学水平研究确定ALA配合小剂量HPD激光光动力疗法促使肿细胞死亡及凋亡的光敏药用药剂量及与二者单独使用的差别方法 :以不同剂量的ALA(40 μg/ml、80 μg/ml、16 0 μg/ml)、HPD(2 .5 μg/ml、5 μg/ml、10 μg/ml)、二者联合与S180腹水瘤细胞一起培养 ,随后以 6 30nm半导体激光 2 0 0mW /cm2 ,照射肿瘤细胞 ,照射 2 0分钟 ,及与不用光敏剂单照激光、单用光敏剂不照光、不用光敏剂不照激光空白对照组比较。以流式细胞仪及倒置微镜观察促使肿瘤细胞凋亡或死亡的差别 ,寻找促使肿瘤细胞凋亡或死亡的最小合适剂量。结果 :ALA4 0 μg/ml配合HPD2 .5 μg/ml与S180腹水瘤细胞同时培养 ,6 30半导体激光 2 0 0mW /cm2 ,照射 2 0分钟组从形态学观察及流式细胞仪测定是能达到较佳的细胞凋亡的最小用光敏剂剂量。单纯ALA -PDT组中能达到小鼠S180腹水瘤细胞明显凋亡的最小剂量是ALA(80 μg/ml) ,单纯HPD -PDT组中能达到小鼠S180腹水瘤细胞明显凋亡最小剂量是HPD(5 μg/ml)。结论 :ALA4 0mg/kg配合HPD2 .5mg/kg ,6 30半导体激光2 0 0mW /cm2 ,照射 2 0分钟是能达到小鼠S180腹水瘤细胞明显凋亡的最小光敏剂剂量。
OBJECTIVE: To determine the dose of photosensitizer and the dose of ALA combined with low-dose HPD laser photodynamic therapy to promote the death and apoptosis of swollen cells and the difference between the two methods by using ALA (40 μg / ml, 80 μg / ml, 160 μg / ml), HPD (2.5 μg / ml, 5 μg / ml, 10 μg / ml) were combined with S180 ascites tumor cells, 0 0mW / cm2, irradiated tumor cells, irradiation 20 minutes, and with no photosensitizer single-shot laser, photosensitizer alone did not light, no photosensitizer without light blank control group comparison. To observe the difference of apoptosis or death of tumor cells by flow cytometry and inverted micromirror and find out the minimum suitable dosage to promote tumor cell apoptosis or death. Results: ALA4 0 μg / ml and HPD2.5 μg / ml were cultured with S180 ascites tumor cells simultaneously. The morphology and flow cytometry of ALA4 0 micromol / The preferred minimum dose of photosensitizer for apoptosis. The minimal dose of ALA (80 μg / ml) that could reach the apoptosis of mouse S180 ascites tumor cells in the ALA-PDT group was the lowest, which was the lowest in HPD-PDT group (5 μg / ml). Conclusion: ALA4 0mg / kg with HPD2 .5mg / kg, 630 semiconductor laser 200mW / cm2, irradiation 20min is the minimum photosensitizer dose that can achieve significant apoptosis of mouse S180 ascites tumor cells.