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分别克隆番茄PR-1和PR-5基因片段,并以之制备探针,采用Northern blot方法研究了PR-1和PR-5基因在正常生长条件下番茄根、茎、叶、果实发育中的表达模式,研究了干旱、复水和内外源乙烯对这两种基因表达的影响.结果表明:这两个基因主要在衰老叶片、B期果实和根部表达,干旱抑制了这两个基因的表达,复水和乙烯诱导其表达.
The PR-1 and PR-5 gene fragments of tomato were cloned and used to prepare probes. Northern blotting was used to study the effects of PR-1 and PR-5 genes on the development of roots, stems, leaves and fruits under normal growth conditions The results showed that these two genes were mainly expressed in the senescence leaves, B fruits and roots, and drought inhibited the expression of these two genes , Rehydration and ethylene induced its expression.