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目的 分析女性生殖道人乳头状瘤病毒(HPV)感染及其基因分型与宫颈癌的关系. 方法 纳入宫颈癌及宫颈癌前病变患者共150例,其中宫颈癌患者63例(宫颈癌组),宫颈癌前病变患者87例(癌前病变组),另选宫颈炎患者50例作为对照组;分析各组患者HPV感染情况,并进行HPV基因分型检测. 结果 200例宫颈疾病患者HPV总阳性率为72.0％(144/200),其中宫颈癌组和癌前病变组HPV阳性率分别为100.0％和67.8％,均显著高于对照组(44.0％)(P＜0.05),且宫颈癌组HPV阳性率明显高于癌前病变组(P＜0.05);宫颈癌患者主要感染HPV16、18、33和58,CIN Ⅰ患者主要感染HPV6、11、16、33、52和59,CINⅡ患者主要感染HPV6、11、16、3I、33、52和58,CINⅢ患者主要感染HPV16、18、31、33、51和58,对照组患者主要感染HPV6、11、33、43、52、58、66及68;宫颈癌组和癌前病变组高危型HPV阳性率(100.0％、77.3％)均显著高于对照组(42.6％)(P＜0.05),且宫颈癌组高危型HPV阳性百分比高于癌前病变组(P＜0.05);宫颈癌组、癌前病变组及对照组单一型HPV感染率分别为100.0％、81.4％和40.9％,多重型HPV感染率分别为0、18.6％和59.1％;HPV感染及高危型HPV感染均与宫颈癌的发生呈正相关(P＜0.05). 结论 宫颈癌的发生与高危型HPV感染密切相关,HPV基因分型检测对宫颈癌前病变及宫颈癌的筛查、诊断及预防具有重要意义.“,”Objective To analyze the relationship between human papilloma virus (HPV) infecting the female genital tract and its genotypes and cervical cancer.Methods Subjects were 150 patients with cervical cancer or precancerous lesions of the uterine cervix.Sixty-three patients had cervical cancer while 87 had precancerous lesions.Fifty patients with cervicitis served as the control group.HPV infection was analyzed in each group and HPV was genotyped.Results The 200 patients tested positive for HPV at a rate of 72.0％ (144/200).Patients with cervical cancer tested positive for HPV at a rate of 100.0％ and patients with precancerous lesions tested positive at a rate of 67.8％.Both groups tested positive for HPV at a significantly higher rate than the control group (44.0％) (x2 =46.902,7.452,P＜0.05).Patients with cervical cancer tested positive for HPV at a higher rate than patients with precancerous lesions did (x2 =24.929,P＜0.05).Patients with cervical cancer were mainly infected with HPV16,18,33,or 58.Of the patients with precancerous lesions,those with CIN Ⅰ lesions were mainly infected with HPV6,11,16,33,52,or 59.Patients with CIN Ⅱ lesions were mainly infected with HPV6,11,16,31,33,52,or 58.Patients with CIN Ⅲ lesions were mainly infected with HPV16,18,31,33,51,or 58.Patients in the control group were mainly infected with HPV6,11,33,43,52,58,66,or 68.Patients with cervical cancer (100.0％) and patients with precancerous lesions (77.3 ％) tested positive for high-risk HPV at a significantly higher rate than the control group did (42.6％) (x2 =47.965,19.058,P＜ 0.05).Patients with cervical cancer tested positive for high-risk HPV at a higher rate than patients with precancerous lesions did (x2=12.154,P＜0.05).The rate of single HPV infection was 100.0％ in patients with cervical cancer,81.4％ in patients with precancerous lesions,and 40.9％ in the control group.The rate of multiple HPV infection was 0.0％ in patients with cervical cancer,18.6％ in patients with precancerous lesions,and 59.1％ in the control group.HPV infection and high-risk HPV infection were correlated with the development of cervical cancer (r=0.423,0.392,P ＜0.05).Conclusion The development of cervical cancer is closely related to infection with high-risk HPV.HPV gen otyping is crucial to screening for,diagnosing,and preventing cervical precancerous lesions and cervical cancer.