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利用玉米自交系黄早四为背景、CML343为供体构建一套在胎萌上存在显著变异的导入系群体为材料,对胎萌的遗传基础进行分析。通过生长箱模拟田间实际环境对该群体授粉后38 d和收获后60 d后的种子发芽率进行测定,以授粉38 d发芽率/收获后60 d的发芽率的比值作为胎萌的指标,结合201对SSR基因型结果,利用GGT2.0软件对胎萌进行QTL分析,共定位了14个控制玉米胎萌的QTL位点,其中两个位点(qVp1-1、qVp1-4)与前人克隆的与脱落酸(ABA)合成有关的基因处于同一染色体区域。
Using maize inbred line Huangzao 4 as background, CML343 was used to construct a set of inbred line population with significant variation in embryonic germplasm as material, and the genetic basis of embryo transfer was analyzed. The seed germination rate of 38 d post-pollination and 60 d post-harvest were measured by growth chamber to simulate the seed germination rate of the population. The ratio of germination rate at 38 d after pollination and the germination rate at 60 d after harvest was taken as the index of embryo germination. 201 pairs of SSR genotypes were analyzed by QG analysis using GGT2.0 software. A total of 14 QTLs controlling maize embryo loci were identified, of which two loci (qVp1-1, qVp1-4) Cloned genes related to abscisic acid (ABA) synthesis are in the same chromosomal region.