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采集了安徽某铁矿酸性矿山废水库周边土壤样品,测定其理化参数,并采用分子生物学方法分别研究了土壤中细菌和古菌群落组成以及驱动氨氧化作用的微生物类群.结果表明,该土壤样品受到酸性矿山废水严重污染,pH低于3,铁铝等金属含量很高.细菌的16S rRNA基因文库覆盖11个类群,其中酸杆菌门(Acidobacteria)占主导地位,丰度达47.4%;疣微菌门(Verrucomicrobia)为第二大门类,丰度为18.9%.样品中含有大量嗜酸的细菌类群,如Candidatus Koribacter versatilis和Holophaga sp.等.古菌的16S rRNA基因文库仅覆盖2个类群,分别为奇古菌门(Thaumarchaeota)和广古菌门(Euryarchaeota),奇古菌门占绝对优势地位.该土壤样品中的氨氧化作用可能主要由奇古菌门的氨氧化古菌所驱动,且该区域存在新的氨氧化古菌类群.
The soil samples collected from an iron mine acidic mine waste water reservoir in Anhui Province were collected and their physical and chemical parameters were determined. The composition of bacteria and archaea community in soil and the microbial groups driving ammoxidation were studied by molecular biology method. The results showed that the soil The samples were seriously polluted by acid mine wastewater, the pH was lower than 3 and the contents of iron, aluminum and other metals were very high.The bacterial 16S rRNA gene library covered 11 groups, of which acidobacteria dominated with an abundance of 47.4% Verrucomicrobia is the second most abundant species with an abundance of 18.9%. The sample contains a large number of acidophilic bacterial groups such as Candidatus Koribacter versatilis and Holophaga sp. The archaeal 16S rRNA gene library covers only two groups , Respectively, Thaumarchaeota and Euryarchaeota, with the dominant genus Myceliophthora.The ammoxidation in this soil sample may be mainly driven by archaea , And there is a new ammoniacal archaeal group in this area.