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实验证明淡水白鲳、尼罗罗非鱼、鲤鱼、革胡子鲶正常生长的水体溶氧重应高于３ｍｇ／ｌ。稻田人工生物圈中水体的溶氧量的日变化规律与池塘相似都为昼高夜低，但人工生物圈水体的溶氧量低于池塘，而且鱼坑中的溶氧量又低于田面，在养殖的后期人工生物圈中水体夜间的溶氧量表现为缺乏。用外源喷水的方式进行增氧，结果表明，在夜间喷水４５ｍｉｎ，喷水量２２５ｍ￣３／ｈｍ￣３，可以满足产量为４３７７．２ｋｇ／ｈｍ￣２的稻田人工生物圈中鱼类对溶氧的要求。而且，外源喷水系统的增氧效果与水体中鱼类的密度成反比。在相同鱼种及放养比例的条件下，有喷水的人工生物圈的鱼产量是不喷水的高密度养鱼的２倍，且成活率高、个体较大。
Experiments show that freshwater pomfret, Nile tilapia, carp, catfish grow normally dissolved oxygen weight should be higher than 3mg / l. Diurnal variation of dissolved oxygen in the water bodies in paddy field artificial biosphere was similar to that in ponds, but the dissolved oxygen in artificial biosphere water bodies was lower than that in ponds, and the dissolved oxygen content in fish pits was lower than that in the field. The late artificial biosphere in the body of oxygen dissolved oxygen showed a lack of. The results showed that spraying water at night for 45min and spraying water amount of 225m ~ 3 / hm ~ 3 could meet the demand of fish in the artificial biosphere of paddy field with the yield of 4377.2kg / hm ~ 2 Dissolved oxygen requirements. Moreover, the exogenous water injection system of oxygen effect and the density of fish in water is inversely proportional. Under the same species and stocking ratio, the artificial biosphere with water jets is twice as productive as high-density fish that is not sprayed and has a high survival rate and a large individual.