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目的:观察靶向沉默Gankyrin基因对结肠癌细胞SW620在增殖和侵袭方面的影响。方法:通过体外合成针对Gankyrin基因的siRNA,通过脂质体转染SW620细胞,设立对照组检测细胞增殖、凋亡、细胞周期及侵袭能力。结果:细胞转染后,实验组的倍增时间为25.6±0.8小时,对照组倍增时间为21.7±0.6小时(P<0.01);细胞周期检测实验组的G1期细胞为68.3±0.7、S期细胞为29.6±0.9,对照组的G1期细胞为44.1±0.3、S期细胞为4.8±0.4(P<0.01);细胞凋亡检测实验组AnnexinV+PI-细胞比例为8.93±0.21,明显高于对照组1.31±0.06(P<0.01);细胞侵袭实验实验组细胞侵袭能率为34.2±0.3,明显低于对照组的53.9±0.6(P<0.01)。结论:siRNA干扰Gankyrin基因表达能够明显降低SW620的增殖和侵袭能力,提示Gankyrin可作为结肠癌基因治疗的潜在靶点。
OBJECTIVE: To observe the effect of targeted silencing of Gankyrin on the proliferation and invasion of colon cancer cell line SW620. Methods: siRNA against Gankyrin gene was synthesized in vitro and transfected into SW620 cells by lipofectamine. The control group was established to detect cell proliferation, apoptosis, cell cycle and invasion. RESULTS: After transfection, the doubling time was 25.6 ± 0.8 hours in the experimental group and 21.7 ± 0.6 hours in the control group (P <0.01). The G1 phase cells in the cell cycle assay group were 68.3 ± 0.7, S phase Cells in the control group were 44.1 ± 0.3 in the G1 phase and 4.8 ± 0.4 in the S phase (P <0.01). The ratio of Annexin V + PI-cells in the apoptosis assay group was 8.93 ± 0.21 , Which was significantly higher than that of the control group (1.31 ± 0.06, P <0.01). The invasion rate of cell invasion experiment group was 34.2 ± 0.3, which was significantly lower than that of the control group (53.9 ± 0.6, P <0.01). CONCLUSIONS: siRNA interference with Gankyrin gene expression can significantly reduce the proliferation and invasion ability of SW620, suggesting that Gankyrin may be a potential target for gene therapy of colon cancer.