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目的 探讨细胞周期素D1(CD1)与大肠癌的发生、进展及预后的关系。方法 收集 2 4例手术切除的大肠癌标本 ,同时取距癌灶 5cm以外的癌旁组织及 10cm以外的正常组织。用原位杂交的方法检测癌组织、癌旁组织及正常组织中CD1mRNA的表达 ,同时结合临床病理资料进行分析。结果 2 4例大肠癌组织中CD1mRNA阳性 9例 ,阳性率 37 5 % ;癌旁组织仅有 1例阳性 ,阳性率 4 2 % ;正常组织未见阳性表达。癌旁组织和正常组织均与癌组织有显著性差异 (P <0 0 1)。CD1mRNA的表达在浸润至浆膜层组明显高于浸润至肌层组 (P <0 0 5 ) ;有淋巴结转移组及晚期病变 (DukesB +C +D期 )组亦明显高于无淋巴结转移及早期病变(DukesA期 )组 (P <0 0 5 )。CD1mRNA的表达高低与年龄、性别、癌灶直径大小、分化程度及是否有远处转移无明显关系 (P >0 0 5 )。结论 CD1mRNA过度表达与大肠癌的发生、发展及转移有关。
Objective To investigate the relationship between cyclin D1 (CD1) and the occurrence, progression and prognosis of colorectal cancer. Methods Twenty-four patients with colorectal cancer who underwent surgical resection were collected. At the same time, cancerous tissues 5 cm away from the tumor and normal tissues other than 10 cm were taken. The in situ hybridization was used to detect the expression of CD1 mRNA in cancerous tissues, paracancerous tissues and normal tissues. The clinical pathological data were used for analysis. Results Of the 24 cases of colorectal cancer, 9 cases were positive for CD1 mRNA, and the positive rate was 37.5%. There was only 1 case in the adjacent tissue. The positive rate was 42%; no positive expression was found in normal tissues. There was a significant difference between paracancerous and normal tissues and cancer tissues (P < 0 01). The expression of CD1 mRNA was significantly higher in the infiltrating into the serosal layer than that in the muscular layer (P < 0.05). The lymph node metastasis group and the late-stage disease (DukesB + C + D phase) group were also significantly higher than those without the lymph node metastasis. Early stage disease (Dukes A stage) group (P <0 05). The expression of CD1 mRNA was not significantly associated with age, sex, size of tumor, differentiation, and distant metastasis (P > 0.05). Conclusion The overexpression of CD1 mRNA is related to the occurrence, development and metastasis of colorectal cancer.