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目的:考察葡萄内酯对脑缺血致血管性痴呆大鼠的保护作用。方法:SD大鼠随机分成假手术组、模型组、安理申组(1.028 mg.kg-1)和葡萄内酯低、中、高剂量(0.257,0.514,1.028 mg.kg-1)组,ig给药,连续52 d。采用永久性结扎大鼠双侧颈总动脉法建立血管性痴呆模型,以Morris水迷宫试验进行行为学测试,观察大鼠的学习记忆能力,检测大鼠脑组织中乙酰胆碱酯酶(AChE)和超氧化物歧化酶(SOD)的活性以及丙二醛(MDA)和谷氨酸含量的变化。结果:中、高剂量葡萄内酯可以明显缩短大鼠的逃避潜伏期,提高其穿越平台次数,增强大鼠的空间学习记忆能力,与模型组比较有极显著性差异(P<0.01)。葡萄内酯各剂量均能降低血管性痴呆大鼠AChE活性以及MDA和谷氨酸含量,与模型组相比,均有显著性差异(P<0.01)。葡萄内酯中、高剂量组的SOD活性明显低于模型组(P<0.01)。结论:葡萄内酯对血管性痴呆有保护作用,其作用机制可能与其降低MDA含量、乙酰胆碱酯酶活性以及谷氨酸对脑神经的毒害作用有关。
Objective: To investigate the protective effect of grape lactone on vascular dementia induced by cerebral ischemia in rats. Methods: SD rats were randomly divided into sham operation group, model group, Angstrom group (1.028 mg.kg-1) and low, medium and high doses of lactone (0.257, 0.514 and 1.028 mg.kg- Administration, for 52 consecutive days. Vascular dementia model was established by permanent ligation of bilateral common carotid arteries in rats. Morris water maze test was used to conduct behavioral testing. The learning and memory ability of rats was observed. AChE and AChE The activity of SOD and the content of malondialdehyde (MDA) and glutamic acid. Results: Middle and high dosage of Lactone can significantly shorten the escape latency of rats, increase the number of crossing the platform and enhance the spatial learning and memory ability of rats. Compared with the model group, there is a significant difference (P <0.01). Compared with the model group, all doses of VE could decrease the activity of AChE and the contents of MDA and glutamate in vascular dementia rats (P <0.01). The activity of SOD in medium and high dose group was significantly lower than that in model group (P <0.01). CONCLUSION: Vitis lactone has a protective effect on vascular dementia. Its mechanism may be related to the decrease of MDA content, the activity of acetylcholinesterase and the toxic effect of glutamate on the cranial nerves.