论文部分内容阅读
将电泳与羟基磷灰石色谱技术耦合 ,开发了一种新的电泳色谱技术。建立了羟基磷灰石 (HAP)吸附牛血清白蛋白 (BSA)的物理模型和数学模型 ,建立了不同的实验装置以研究电场下 HAP吸附 BSA的反应动力学特性以及电动色谱吸附 BSA过程的传质动力学特性。实验结果表明 ,施加电场不会改变吸附反应的平衡常数与速率 ,但在电泳羟基磷灰石色谱装置中 ,施加电场在羟基磷灰石颗粒表面产生的电渗流能够加强扰动 ,减少液膜扩散阻力 ,提高羟基磷灰石色谱的动态吸附容量
Coupled electrophoresis and hydroxyapatite chromatography, a new electrophoretic chromatography technique was developed. The physical and mathematical models of hydroxyapatite (HAP) -based bovine serum albumin (BSA) were established. Different experimental setups were set up to study the reaction kinetics of BSA adsorbed by HAP under electric field, as well as the kinetics of BSA adsorption by electrokinetic chromatography Qualitative dynamic properties. The experimental results show that the application of electric field does not change the equilibrium constant and the rate of adsorption reaction. However, in the electrophoresis hydroxyapatite chromatography device, the electroosmotic flow generated by the applied electric field on the surface of hydroxyapatite particles can enhance the perturbation and reduce the diffusion resistance , To improve the dynamic adsorption capacity of hydroxyapatite chromatography