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建立了洛美沙星的化学发光-酶联免疫分析方法。实验对包被抗原的度盘浓度、洛美沙星抗体及辣根过氧化物酶标记的羊抗兔二抗的稀释倍数、酶标板的种类等进行了优化。在最佳实验条件下,测得的IC50值为0.22ng/mL,检测限为0.032 ng/mL。该方法具有良好的特异性,在检测的10种代表性的喹诺酮类药物中,仅诺氟沙星和氟罗沙星交叉率高于1%(分别为:6.88%和2.16%)。将该方法应用于脱脂牛奶中添加洛美沙星回收率的测定,批内和批间回收率分别为:88.7%～103.4%和97.8%～107.2%,相应变异系数分别为:6.3%～8.6%和2.4%～8.0%。
A chemiluminescence-enzyme-linked immunosorbent assay was developed for the determination of lomefloxacin. Experiments on the coated plate concentration of antigens, lomefloxacin antibody and horseradish peroxidase labeled goat anti-rabbit secondary dilution times, the type of ELISA plate was optimized. Under the best experimental conditions, the IC50 value was 0.22ng / mL and the detection limit was 0.032 ng / mL. This method has good specificity. Of the 10 quinolones tested, only crossover rates of norfloxacin and fleroxacin were above 1% (6.88% and 2.16%, respectively). The method was applied to the determination of the recoveries of lomefloxacin in skimmed milk. The recoveries were between 88.7% and 103.4% and between 97.8% and 107.2%, respectively. The corresponding coefficients of variation were 6.3% -8.6% And 2.4% ~ 8.0%.