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目的采用5%牛磺胆酸钠逆行胰胆管注射建立急性出血坏死性胰腺炎(AHNP)大鼠模型,动态观察肺组织损伤情况,以评价能否建立有效的急性胰腺炎相关性肺损伤(APALI)动物实验模型。方法SD大鼠随机分成假手术对照组和AHNP模型组,动态观察3,6,12和24h四个时相点的血清淀粉酶、胰腺和肺病理组织学改变、肺组织湿干重比和髓过氧化物酶活性,伊文氏蓝法观察1.5,3和6h三个时相点肺血管通透性。结果血清淀粉酶、肺组织湿干重比和髓过氧化物酶活性在5%牛磺胆酸钠诱导后3h明显增高,并持续到24h,肺组织病理检查发现,牛磺胆酸钠诱导后肺组织就出现了明显的充血、出血、炎症细胞浸润、组织结构破坏等肺组织损伤的病理表现,AHNP大鼠1.5h肺血管通透性就显著增高,且随时间呈显著性增加,各指标与假手术组比较差异有显著性(P<0.05)。结论应用5%牛磺胆酸钠能够成功建立APALI大鼠模型,为进一步研究对APALI的防治作用奠定了基础。
Objective To establish a rat model of acute hemorrhagic necrotizing pancreatitis (AHNP) by injecting 5% sodium taurocholate into the pancreaticobiliary duct and observe the damage of the lung tissue dynamically to evaluate whether an effective acute pancreatitis associated lung injury (APALI) Animal experiment model. Methods SD rats were randomly divided into sham operation control group and AHNP model group. Dynamic changes of serum amylase, pancreas and lung histopathological changes at 3, 6, 12 and 24 h were observed. Peroxidase activity and pulmonary vascular permeability at three time points of 1.5, 3 and 6 h were observed by Evan’s blue method. Results Serum amylase, wet-to-dry weight ratio and myeloperoxidase activity in lung tissue were significantly increased 3h after induced by 5% sodium taurocholate and lasted for 24 h. Pathological examination of lung tissue showed that after induced by sodium taurocholate Lung tissue showed obvious hyperemia, hemorrhage, inflammatory cell infiltration, tissue damage and other pathological lung tissue damage, AHNP 1.5h pulmonary vascular permeability was significantly increased, and with time was significantly increased, the various indicators Compared with the sham operation group, the difference was significant (P <0.05). Conclusion The APALI rat model can be successfully established by using 5% sodium taurocholate, which lays the foundation for further study on the prevention and treatment of APALI.