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目的:探讨神经毒素6 -羟基多巴(6 - OHDA)在帕金森病(PD)发病机制中的作用。方法:采用立体定向术将神经毒素6 - OHDA注入大鼠右侧纹状体内,制备经典的帕金森病动物模型。造模2个月后,检测其纹状体内还原型谷胱甘肽(GSH)和活性氧,并观察黑质的病理改变。结果:帕金森病模型组右侧纹状体内GSH含量[(36 .85±8.6 4 μg·m g- 1 prot) ]明显下降,活性氧含量[(5 8.6 9±9.84 ) U·m g- 1 prot]明显增高,与假手术组及正常对照组比较差异均有显著性(P<0 .0 5 )。光镜下可见帕金森病模型组右侧黑质致密带内多巴胺能神经元减少,与模型组左侧及对照组、假手术组黑质相比差异均有显著性(P<0 .0 5 )。结论:6 - OHDA可通过耗竭GSH及增加自由基的生成损害多巴胺能神经末梢,并逆行性损毁神经元胞体,导致黑质致密带多巴胺能神经元变性死亡。
Objective: To investigate the role of neurotoxin 6 - hydroxydopamine (6 - OHDA) in the pathogenesis of Parkinson ’s disease (PD). Methods: The stereotactic technique was used to inject neurotoxin 6 - OHDA into the right striatum of rats to prepare a classic animal model of Parkinson ’s disease. After 2 months of modeling, the reduced glutathione (GSH) and reactive oxygen species (ROS) in the striatum were detected and pathological changes of substantia nigra were observed. Results: The GSH level in the right striatum [(36.85 ± 8.64 μg · m g-1 prot)] in Parkinson’s disease model group decreased significantly, and the content of reactive oxygen species [(5 8.69 ± 9.84) U · m g- 1 prot] was significantly higher than that in sham operation group and normal control group (P <0.05). The number of dopaminergic neurons in substantia nigra compact zone on the right side of Parkinson’s disease model group was significantly lower than that on the left side of the model group and the control group and sham operation group (P <0.05) ). CONCLUSION: 6 - OHDA can damage dopaminergic nerve endings by depleting GSH and increasing the production of free radicals, and can retrograde damage the neuronal soma, resulting in the degeneration of dopaminergic neurons in substantia nigra.