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探讨常氧与缺氧状态下脑啡肽对兔肺动脉平滑肌增殖的调控作用及机制 ,为解决经皮腔内冠脉成形术 (PTCA)支架后冠脉再狭窄问题提供新的理论依据。无菌取出新西兰白兔肺动脉段 ,在常氧和缺氧条件下按照 Ross贴片法培养平滑肌 ,采用相差显微镜、透射电镜和 (-肌动蛋白抗体染色法鉴定平滑肌细胞 ,四唑盐比色法及 3H- Td R参入实验检测平滑肌的生长状况。单纯缺氧明显促进平滑肌细胞的增殖 ;常氧和缺氧状态下脑啡肽显著抑制平滑肌的增生 ;吗啡对平滑肌的生长无明显影响 ;纳洛酮能拮抗常氧和缺氧状态下脑啡肽抑制平滑肌的增殖调控作用。 PTCA支架后冠脉再狭窄的发生可能与 PTCA支架后机体血浆中脑啡肽水平的降低和冠脉新生内膜平滑肌细胞的缺氧有关
To investigate the regulatory effect of enkephalin on proliferation of pulmonary artery smooth muscle in rats under normoxia and hypoxia and to provide a new theoretical basis for resolving coronary stenosis after percutaneous transluminal coronary angioplasty (PTCA). Pulmonary arterial segments of New Zealand white rabbits were aseptically removed and cultured under the condition of normoxia and anaerobic. The smooth muscle cells were cultured by Ross patch method. The smooth muscle cells were identified by phase contrast microscopy, transmission electron microscopy and (- actin antibody staining, tetrazolium salt colorimetry And 3H-TdR were involved in the experiment to detect the growth of smooth muscle.Induced hypoxia obviously promoted the proliferation of smooth muscle cells; Eno and hypoxia enkephalin significantly inhibited the proliferation of smooth muscle; morphine had no effect on smooth muscle growth; Ketone can antagonize the inhibitory effect of enkephalin on proliferation and proliferation of smooth muscle cells under normoxia and hypoxia.Coronary artery restenosis after PTCA stent may be related to the decrease of plasma midbrain enkephalin level and coronary neointimal smooth muscle Cell hypoxia