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目的:研究果糖二磷酸钠(FDP)对2型糖尿病(T2DM)大鼠周围神经病变的影响。方法:首先建立T2DM大鼠模型,将实验动物分成4组,正常对照组、模型对照组、FDP高剂量治疗组(5mL.kg-1.d-1,i.p.)和FDP低剂量治疗组(2mL.kg-1.d-1,i.p.),每组8只。8周末,测定大鼠尾运动神经传导速度(MCV)、感觉神经传导速度(SCV),H反射中H与M波的潜伏期,血清血糖(FBG)与超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)的变化。结果:①与正常组比较,FDP干预前,T2DM大鼠FBG、血清胰岛素(FINS)水平显著升高,胰岛素敏感指数(ISI)显著降低(P<0.01);②FDP干预后,与正常组比较,模型对照组FBG、FINS水平显著升高,ISI、SOD、NOS和MCV、SCV显著下降,H与M波潜伏期显著延长(P<0.01);③与模型对照组比较,FDP干预后,高剂量组大鼠FBG、FINS水平显著降低,ISI、SOD、NOS、MCV和SCV显著升高,H与M波潜伏期显著缩短(P<0.01);低剂量组大鼠FBG显著降低,SOD、NOS、MCV和SCV显著升高,M波潜伏期显著缩短(P<0.01或P<0.05);④与低剂量治疗组比较,高剂量组大鼠FBG、FINS水平显著降低,ISI、SOD、NOS、MCV和SCV显著升高,H与M波潜伏期显著缩短,(P<0.01或P<0.05)。结论:FDP对糖尿病大鼠周围神经病变具有一定的治疗作用,其机制可能与FDP降低糖尿病大鼠FBG和提高机体抗氧化能力有关。
Objective: To study the effect of fructose diphosphate (FDP) on peripheral neuropathy in type 2 diabetes mellitus (T2DM) rats. Methods: The T2DM rat model was established. The experimental animals were divided into 4 groups: normal control group, model control group, FDP high dose treatment group (5mL.kg-1.d-1, ip) and FDP low dose treatment group kg-1.d-1, ip), 8 in each group. At the end of 8th week, MCV, SCV, latency of H and M waves in H reflex, serum glucose (FBG) and superoxide dismutase (SOD), nitric oxide Nitrogen synthase (NOS) changes. Results: ①Compared with the normal group, the level of FBG and FINS in T2DM rats significantly increased and the insulin sensitivity index (ISI) decreased significantly (P <0.01) before intervention with FDP; ②Compared with the normal group, The levels of FBG and FINS in model control group were significantly increased, while the levels of ISI, SOD, NOS, MCV and SCV were significantly decreased (P <0.01); ③ Compared with model control group, The levels of FBG and FINS were significantly decreased, the ISI, SOD, NOS, MCV and SCV were significantly increased, while the latency of H and M wave was significantly shortened (P <0.01) (P <0.01 or P <0.05). ④ Compared with the low-dose treatment group, the levels of FBG and FINS in the high-dose group were significantly decreased and the levels of ISI, SOD, NOS, MCV and SCV were significantly increased (P <0.01 or P <0.05). The latency of H and M waves was significantly shortened. Conclusion: FDP has a certain therapeutic effect on peripheral neuropathy in diabetic rats. The mechanism may be related to the effect of FDP on reducing FBG and improving the antioxidant capacity in diabetic rats.