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植物应答逆境的防御反应在很大程度是在基因转录水平调节的,其中转录因子与目标基因启动子上顺式作用元件的识别和结合起着关键的调节作用.本研究将从双子叶植物中发现的GCC盒(应答乙烯)和JERE盒(应答JA和激发子)与CaMV35S核心启动子融合构建成诱导型启动子,利用GUS报告基因构建其表达载体并进行农杆菌介导的水稻遗传转化.利用T1代株系分析了GCC盒和JERE盒水稻植株对不同逆境胁迫和激素处理的应答.结果表明在转基因植株中它们都具有很低的本底表达.稻瘟病侵染、稻纵卷叶螟取食和机械损伤处理可不同程度提高GUS基因的表达.另外,脱落酸(ABA)、水杨酸(SA)和茉莉酸(JA)也能提高GUS的表达.实验结果暗示JERE和GCC盒介导的信号途径在单子叶和双子叶之间有一定的保守性.图5参15
The defensive response of plants to stress response is to a large extent regulated at the level of gene transcription, in which transcription factors play a key regulatory role in the recognition and binding of cis-acting elements on the promoter of the target gene.In this study, The found GCC box (response ethylene) and JERE box (response JA and elicitor) were fused with the CaMV35S core promoter into an inducible promoter. The GUS reporter was used to construct its expression vector and Agrobacterium-mediated rice genetic transformation. The T1 generation lines were used to analyze the response of GCC box and JERE box rice plants to different stresses and hormone treatments.The results showed that all of them had low background expression in the transgenic plants.The rice blast infection, In addition, abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) also enhanced the expression of GUS.The results of the experiment indicated that JERE and GCC box mediated The signal transduction pathways are somewhat conserved between monocotyledons and dicotyledons