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【目的】考察菌株Trichosporon montevideense WIN合成纳米金的催化特性及应用。【方法】利用活性WIN菌作用不同浓度HAu Cl_4(1、2和4 mmol/L)合成纳米金的特性,分别利用活性WIN菌和灭活WIN菌合成纳米金,分析合成纳米金的形貌、粒径及其催化特性。【结果】HAu Cl_4浓度为1 mmol/L时,菌株WIN合成了纳米金,HAu Cl_4浓度为2 mmol/L和4 mmol/L时,菌株WIN合成了纳米金及较大尺寸的金颗粒。通过紫外-可见光谱扫描、透射电子显微镜分析,发现活性和灭活WIN菌均能还原Au~(3+)合成纳米金,合成的纳米金均以球形为主,还有少量三角形、四边形及六边形。活性WIN菌合成的纳米金粒径范围为3 nm-252 nm,平均粒径为45.2 nm,而灭活WIN菌合成的纳米金为1 nm-271 nm,平均粒径为38.3 nm。活性和灭活WIN菌合成的纳米金对还原4-硝基苯酚的催化速率分别为2.76×10~(-3)s~(-1)和4.84×10~(-3)s~(-1)。【结论】菌株Trichosporon montevideense WIN的活性及灭活细胞均可以合成纳米金,且合成的纳米金具有良好的催化特性,在催化去除环境中难降解污染物中具有一定的应用前景。
【Objective】 To investigate the catalytic properties of Trichosporon montevideense WIN and its application. 【Method】 The characteristics of gold nanoparticles synthesized by different concentrations of HAuCl_4 (1, 2 and 4 mmol / L) were studied by using active Wister strain. The gold nanoparticles were synthesized by active and inactivated Wins, Particle size and its catalytic properties. 【Result】 The results showed that when HAu Cl 4 was 1 mmol / L, gold nanoparticles were synthesized by the strain WIN and gold nanoparticles and larger gold particles were synthesized by the strain WIN at concentrations of 2 mmol / L and 4 mmol / L HAu Cl 4. By UV-Vis spectroscopy and transmission electron microscopy analysis, it was found that the active and inactivated Wisteria can all reduce the Au 3+ Au nanostructures. The synthesized Au nanocrystals were mainly spherical with few triangles, tetragons and hexa Polygon. The average particle diameter of Au nanoparticles grown by active WIN strain ranged from 3 nm to 252 nm, and the average particle size was 45.2 nm. The gold nanoparticles synthesized by inactivated Wistar were 1 nm-271 nm with an average particle size of 38.3 nm. The catalytic rates of 4-nitrophenol reduction by the gold nanoparticles synthesized by the activated and inactivated Wistar strains were 2.76 × 10 -3 s -1 and 4.84 × 10 -3 s -1 respectively ). 【Conclusion】 The activity of Trichosporon montevideense WIN and the inactivated cells both can synthesize gold nanoparticles. The synthesized gold nanoparticles have good catalytic properties and have certain application prospects in the catalytic removal of refractory pollutants in the environment.