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With specific designed prmers. CYP2B6 and CYP1A1 cDNA were generatecl by reverse transcrlI7tion-Polymerase chain reaction(RT-PCR )technlque Performed on total RNAs isolated frorn hum1ln liver and 3-rnethylch(,lanthrene(3-Mtt)induc human amnion FL, cells. Cell llnes (CHL, 2B6 and CtHL-1A1 ) capableof expressing hunlan cytochome P 15O (CYP ) 2B6 and 1A1 were establishecl after transfection of corre-sponding eukaryotic reconlbinant expression plasmid with human CYP2ll6 and 1A1 cDNA lnserts respectlvely. These cell lines stably expressed the mRNAs and the enzymatic activltles cc)rresI’onding to ttYP2B6and CYP1A1, respectively’ Compared with Chinese hamster lung (CHL) cells, the n1icr()nucleus frecluencyin CHl,-2B6 cells is markedly lncreased when exPosed to nitrosamines,aflatoxln B, (AFB1) and cyclophos-Phamide (CPA). Thls is also in CHL-1A1 cells,when exposed to carcinogenic polycycllc aromatic hydrocar-bons.
CYP2B6 and CYP1A1 cDNA were generated by by reverse transcrlI7tion-Polymerase chain reaction (RT-PCR) technlque Performed on total RNAs isolated from frorn hum1ln liver and 3-rnethylch (, lanthrene (3-Mtt) inducn Human amnion FL, cells These cells lines stably expressed (CYP) 2B6 and 1A1 were establishecl after transfection of corre-sponding eukaryotic reconlized expression plasmid with human CYP2116 and 1A1 cDNA lnserts respectlly. the mRNAs and the enzymatic activators cc) rlI’onding to ttYP2B6 and CYPlA1, respectively ’Compared with Chinese hamster lung (CHL) cells, the nlicr () nucleus frecluencyin CHl, -2B6 cells is markedly lncreased when exPosed to nitrosamines, aflatoxln B, ( AFB1) and cyclophos-Phamide (CPA). Thls is also in CHL-1A1 cells, when exposed to carcinogenic polycycllc aromatic hydrocar- bons.