论文部分内容阅读
目的前期研究表明,硫磺熏蒸对菊花的化学成分具有显著影响,本实验在此基础上对硫熏前后菊花入肠吸收成分进行比较研究。方法采用肠囊外翻模型,并结合超高压液相色谱-四级杆飞行时间质谱(UPLC-QTof-MS)和HPLC技术研究硫磺熏蒸对菊花入肠吸收成分影响。结果采用UPLC-QTof-MS定性比较,结果发现,硫磺熏蒸导致菊花入肠吸收的成分种类减少,比例发生改变。菊花硫熏前后,肠吸收液中黄酮苷类及黄酮苷元类成分存在显著性差异,硫熏菊花肠液中黄酮苷类成分的种类明显减少。采用HPLC对差异较大的7种成分进行半定量比较。菊花硫熏后,肠吸收液中黄酮苷类成分含量明显降低,而黄酮苷元类成分虽然在硫熏后药材中的含量增加,但在肠吸收液中其含量并未见明显增加。结论菊花经过硫熏后导致药材中化学成分的组成及比例发生变化,从而影响其入肠吸收。
Objective The previous studies showed that sulfur fumigation had a significant effect on the chemical constituents of chrysanthemum. Based on this study, the absorption components of chrysanthemum into the intestine before and after sulfur smoked were comparatively studied. Methods The gut eversion model was used in combination with UPLC-QTof-MS and HPLC to study the effects of sulfur fumigation on the intestinal absorption of chrysanthemum. The results of UPLC-QTof-MS qualitative comparison, the results showed that the sulfur fumigation led to the absorption of chrysanthemum to reduce the types of ingredients, the proportion changed. Chrysanthemum sulfur smoked before and after, intestinal absorption of flavonoid glycosides and flavonoid glycosides have significant differences in elemental composition, sulfur smoked chrysanthemum intestinal flavonoids glycosides in the species was significantly reduced. Semi-quantitative comparison of the seven components, which differ greatly by HPLC, was performed. After chrysanthemum smoked, the content of flavonoid glycosides in intestinal absorption fluid decreased obviously, while the content of flavonoid aglycone did not increase significantly in intestinal absorption fluid even though the content of flavonoid aglycone increased in medicinal materials after sulfur smoked. Conclusion Chrysanthemum after sulfur smoked lead to changes in the composition and proportion of the chemical composition, thus affecting its intestinal absorption.