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目的:通过星点设计-响应面法优化苍术与厚朴的醇提工艺,并联合正交试验优化陈皮与甘草的水提工艺。方法:以乙醇体积分数、提取时间、溶媒比为自变量,以苍术素、厚朴酚及和厚朴酚含量、浸膏得率的总评“归一值”为因变量,通过对自变量各水平进行多元线性回归及二项式拟合,采用响应面法优选提取工艺,并进行预测分析。采用L9(34)正交试验法,以加水量、提取时间、提取次数为考察因素,以橙皮苷含量及浸膏得率为综合评价指标,优选最佳水提工艺。结果:最佳醇提工艺为13倍量65%乙醇提取3次,每次90min;最佳水提工艺为10倍量水提取3次,每次60min。结论:星点设计-响应面法联合正交试验优选的平胃散提取工艺全面合理可行,可推广于大生产应用。
OBJECTIVE: To optimize the alcohol extraction process of Atractylodes macrocephala and Magnolia officinalis by means of apical design - response surface methodology, and optimize the water extraction process of Citrus rehmanniae and Glycyrrhiza with orthogonal experiment. Methods: The ethanol volume fraction, extraction time and solvent ratio were used as independent variables. The effects of atractylosin, honokiol and honokiol content, total rating of extract yield, “normalized value” The variables were multivariate linear regression and binomial fitting at each level. The response surface method was used to optimize the extraction process and the predictive analysis was carried out. L9 (34) orthogonal test was used to determine the optimal water-extraction process based on the amount of water added, the extraction time and the number of extraction. The optimum extraction conditions were the hesperidin content and the extraction yield. Results: The optimal alcohol extraction process was 13 times the amount of 65% ethanol extracted three times, each 90min; the best water extraction process for 10 times the amount of water extracted three times, each time 60min. Conclusion: The combination of star design - response surface method and orthogonal experiment is more reasonable and feasible for the extraction of Pingwei powder, which can be extended to large-scale production applications.