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目的探讨深圳地区人类免疫缺陷病毒(HIV)阳性人群中人乳头瘤病毒(HPV)感染分型情况,为HIV阳性人群中HPV感染的防治提供依据。方法运用荧光PCR方法和反向斑点杂交技术对HPV阳性患者进行HPV分型检测。结果在HIV感染者中,利用反向点杂交分型方法对40例HPV阳性标本进行HPV基因分型。其中单型感染有18例(45.0%),混合感染有19例(47.5%),共检出16种HPV型别,其中包括11种高危型(16、18、31、33、35、45、52、56、58、68、73型)和5种低危型(6、11、42、44、54型)。在16种型别中,感染率最高的为16型(25.0%),其次为52型(17.5%)、58型(15.0%)。结论感染HIV人群中生殖器部位HPV的感染率较高,在HIV阳性人群中检出性病相关的高危HPV 16/52/58亚型,对HIV阳性人群中HPV感染的防治有重要指导意义。
Objective To investigate the classification of human papillomavirus (HPV) in human immunodeficiency virus (HIV) positive population in Shenzhen and to provide basis for prevention and treatment of HPV infection in HIV positive population. Methods Fluorescent PCR and reverse dot blot hybridization were used to detect HPV type in HPV positive patients. Results In HIV-infected persons, 40 HPV-positive specimens were genotyped by reverse-dot hybridization. There were 18 HPV types (45.0%) and 19 (47.5%) with mixed infection, of which 16 HPV types were detected, including 11 high risk types (16,18,31,33,35,45, 52, 56, 58, 68, 73) and five low-risk types (6, 11, 42, 44, 54). Of the 16 genotypes, 16 (25.0%) had the highest infection rates, followed by 52 (17.5%) and 58 (15.0%). Conclusion HPV infection rate in genital area is high in HIV-infected population. Detection of STD-associated high-risk HPV 16/52/58 subtype in HIV-positive population is important for prevention and treatment of HPV infection in HIV-positive population.