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目的探讨促红细胞生成素(EPO)对新生大鼠缺血缺氧性脑损伤(HIBD)后脑组织中凋亡相关因子及其配体(Fas/Fas L)表达的影响。方法选120只7日龄新生大鼠,随机分为3组,假手术组、缺血缺氧组(模型组)和红细胞生成素组(实验组),设5个时间点:HIBD模型建立后6,12,24,48和72 h。每组于各个时间点分别处死8只大鼠,取脑组织,进行HE染色,观察脑细胞形态学改变,免疫组化染色观察Fas/Fas L的表达。结果模型组,Fas和Fas L的表达于HIBD模型建立后48 h达峰值,至72 h明显下降;与假手术组相比,模型组在各个时间点的Fas和Fas L表达均明显增加(P<0.05)。与模型组相比,实验组的Fas和Fas L的表达在各个时间点均有显著降低(P<0.05)。结论 EPO可通过减少HIBD新生大鼠脑组织中Fas及Fas L的表达,抑制脑组织缺氧缺血损伤后的细胞凋亡。
Objective To investigate the effect of erythropoietin (EPO) on the expression of Fas / Fas ligand and Fas ligand in brain tissue of neonatal rats after hypoxic-ischemic brain damage (HIBD). Methods 120 7-day-old neonatal rats were randomly divided into 3 groups: sham operation group, hypoxia-ischemia group (model group) and erythropoietin group (experimental group). Five time points were established: 6, 12, 24, 48 and 72 h. At each time point, 8 rats were sacrificed at each time point. Brain tissue was taken for HE staining to observe morphological changes of brain cells. The expression of Fas / Fas L was observed by immunohistochemistry. Results The expressions of Fas and Fas L were up-regulated in the model group at 48 h after establishment of HIBD model and significantly decreased at 72 h. Compared with the sham operation group, the expression of Fas and Fas L at each time point increased significantly <0.05). Compared with the model group, the expression of Fas and Fas L in the experimental group decreased significantly at all time points (P <0.05). Conclusion EPO can inhibit the apoptosis of brain tissue after hypoxic-ischemic injury by decreasing the expression of Fas and Fas L in brain tissue of HIBD neonatal rats.