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目的采用先进的样品前处理技术、利用气相色谱-串联质谱法建立14种植物生长调节剂(4-氯苯氧乙酸、2,4-二氯苯氧乙酸、α-萘乙酸、β-萘乙酸、吲哚乙酸、吲哚丁酸、2,4-D乙酯、2,4-D丁酯、萘乙酰胺、整形素、多效唑、烯效唑、激动素、6-苄基腺嘌呤)的高通量检测技术。方法样品经前处理后采用DB-5 MS(30 m×0.25 mm,0.25μm)色谱柱程序升温分离,质谱多反应监测模式(MRM)检测,外标法定量。结果 14种植物生长调节剂在相应的浓度范围具有良好的线性关系(r≥0.997 8),各组分检出限在0.02μg/kg~10.00μg/kg。14种植物生长调节剂加标回收率为54.8%~131.6%,相对标准偏差为2.39%~6.38%。结论建立了14种植物生长调节剂的气相色谱-串联质谱的检测方法。方法快速、准确,检出限、回收率和相对标准偏差均符合国内外对植物生长调节剂残留检测的要求。
Objective To establish 14 plant growth regulators (4-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid, α-naphthaleneacetic acid, β-naphthaleneacetic acid) using advanced sample preparation techniques and gas chromatography-tandem mass spectrometry , Indole acetic acid, indole butyric acid, 2,4-D ethyl ester, 2,4-D butylester, naphthylacetamide, steroids, paclobutrazole, uniconazole, kinetin, 6- benzyladenine High-throughput detection technology. Methods The samples were pretreated with DB-5 MS (30 m × 0.25 mm, 0.25 μm) column temperature program and detected by mass spectrometry multiple reaction monitoring (MRM). Results Fourteen plant growth regulators had a good linear relationship (r ≥ 0.997 8) in the corresponding concentration range, and the detection limits of each component ranged from 0.02 μg / kg to 10.00 μg / kg. The spiked recoveries of 14 plant growth regulators were 54.8% ~ 131.6% with relative standard deviations of 2.39% ~ 6.38%. Conclusion The method for the determination of 14 plant growth regulators by gas chromatography-tandem mass spectrometry was established. The method is rapid, accurate, detection limit, recovery and relative standard deviation are in line with the requirements of plant growth regulator residue testing at home and abroad.