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选择重点组合的F1和F2代材料进行花药培养,结果表明,诱导培养基N_6+2,4-D2mg/L+KT1mg/L及W_(14)+2,4-D2mg/L+KT0.5mg/L较好,分化培养基以1/2MS+KT2mg/L+NAA1mg/L+BA1mg/L较好。花药培养的出愈率、绿苗分化率与材料的基因型有很大关系。
The F1 and F2 materials of the key combinations were selected for anther culture. The results showed that induction medium N_6 + 2,4-D2mg / L + KT1mg / L and W_ (14) +2,4-D2mg / L + KT0.5mg / L better differentiation medium with 1 / 2MS + KT2mg / L + NAA1mg / L + BA1mg / L better. Anther culture rate of emergence, green seedling differentiation rate and the genotype of the material has a great relationship.