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目的通过大鼠孕期不同阶段染铅制备仔鼠染铅模型,测定仔鼠血铅及脑铅水平,并观察S100B蛋白在不同染铅模型仔鼠脑组织中的表达及意义。方法将72只健康清洁级Wistar妊娠大鼠随机分为孕早期染铅组(孕早期:妊娠1～10d饮用0.25g/L醋酸铅溶液;孕后期:妊娠11～20d饮用蒸馏水)、孕晚期染铅组(孕早期饮用蒸馏水,孕后期饮用0.25g/L醋酸铅溶液)、孕全程染铅组(妊娠1～20d饮用0.25g/L醋酸铅溶液)和对照组(孕全程饮用蒸馏水),每组18只。各组妊娠末期剖宫取仔鼠,采血测定血铅水平;完整取出仔鼠大脑,测定脑铅水平和S100B蛋白的表达。结果仔鼠血铅及脑铅水平由低至高依次为对照组、孕晚期染铅组、孕早期染铅组、孕全程染铅组,任意两组比较,差异均有统计学意义(F=12.01、7.39,P<0.05)。与对照组比较,孕期染铅各组子代仔鼠脑组织中S100B蛋白的表达均升高;与孕全程染铅组比较,孕早期及孕晚期染铅组子代仔鼠脑组织中S100B蛋白的表达均下降,差异有统计学意义(F=3.73,P<0.05)。结论孕期铅暴露使得子代仔鼠脑组织中S100B蛋白的表达升高,以孕全期铅暴露组表达水平最高。母孕期低水平铅暴露导致子代仔鼠脑组织中S100B蛋白表达异常可能是铅致脑损伤的毒性机制之一。
OBJECTIVE: To prepare the model of lead exposure in different stages of pregnancy, and to determine the level of blood lead and brain lead in the offsprings, and to observe the expression of S100B protein in the brain tissues of the offspring of different lead-exposed rats. Methods 72 Wistar pregnant rats of healthy and clean grade were randomly divided into the first trimester lead exposure group (first trimester pregnancy: 0.25g / L lead acetate solution during pregnancy 1-10 days; the second trimester pregnancy: 11-20 days’ gestation drinking distilled water) Group (distilled water in the first trimester and 0.25g / L lead acetate solution in the second trimester), lead poisoning in the whole pregnancy (0.25g / L lead acetate solution for 1 ~ 20days pregnancy) and control group . The cesarean sections were taken from the third trimester of pregnancy and the blood lead level was determined by blood sampling. The brain of the offspring was completely removed and the levels of lead and S100B protein were measured. Results The level of blood lead and brain lead levels in the offspring were the control group, the lead exposure group in the second trimester, the lead exposure group in the third trimester, and the lead in the whole pregnancy group. The differences were statistically significant (F = 12.01 , 7.39, P <0.05). Compared with the control group, the expression of S100B protein in the brain tissue of offspring of offspring of pigs during pregnancy were all increased. Compared with the whole lead exposure group, the levels of S100B protein (P <0.05), the difference was statistically significant (F = 3.73, P <0.05). Conclusion Lead exposure during pregnancy increased the expression of S100B protein in the offspring of offspring rats, and reached the highest level in the lead exposure group during pregnancy. Abnormal expression of S100B protein in the offspring of offspring may be one of the mechanisms of lead-induced brain injury.