论文部分内容阅读
稀土在作物上的研究和使用,我国始于1972年。它对作物的发芽、幼苗生长和产量都有很大的促进作用,并影响作物自身和某些物质代谢及其酶活。但稀土元素用于食用菌上,对食用菌生理机制的研究报道较少。本试验采用不同稀土浓度处理香菇培养料,在培养25天、50天、85天、145天取样,测定培养基中三素变化和20ppm浓度下有关酶活变化,结果稀土处理对物质降解有促进作用,能提高营养生长阶段的酶活性。 一、材料与方法 (一)菌种 香菇7402,由本所提供。 (二)试验方法 采用三角瓶培养,培养基采用常规木屑麸皮培养基,在25℃下培养和栽培。 (三)组分分析 在培养0天、25天、50天、85天,145天取样,试验设CK、10ppm、20ppm、30ppm处理,样品60℃烘至恒重,粉碎,60目过筛。测定方法:干样品1.000g,置于碘瓶中,加入70ml中性洗涤剂,于高压锅100℃下保持40分钟,后提高到115℃20分钟,取出用G_3漏斗抽滤,残渣用90℃蒸馏水、95％乙醇、丙酮冲洗数次,将残渣进行真空干燥.
Rare earth in crop research and use, our country began in 1972. It has great effect on crop germination, seedling growth and yield, and affects the crop itself and certain substances metabolism and its enzyme activity. However, the use of rare earth elements for edible fungi, the physiological mechanism of edible fungi reported less. In this experiment, mushrooms were treated with different rare earth concentrations, and samples were taken at 25 days, 50 days, 85 days and 145 days. The changes of three factors in the medium and the changes of enzyme activity at 20 ppm were determined. As a result, Role, can improve the nutritional growth stage of the enzyme activity. First, the material and methods (A) mushroom mushrooms 7402, provided by the Institute. (B) The test method using triangular flask culture medium using conventional sawdust bran culture medium, cultured at 25 ℃ and cultivation. (3) Analysis of Components Samples were taken at 0 days, 25 days, 50 days, 85 days and 145 days of culture. The treatments were set at CK, 10ppm, 20ppm and 30ppm. Samples were dried at 60 ℃ to constant weight, crushed and sieved through 60 mesh. Determination: dry sample 1.000g, placed in an iodine bottle, add 70ml neutral detergent, maintained in a pressure cooker at 100 ℃ for 40 minutes, then raised to 115 ℃ for 20 minutes, removed by G_3 funnel suction filtration, the residue was distilled water 90 ℃ , 95% ethanol, acetone rinse several times, the residue was vacuum dried.