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快速叶绿素荧光动力学可以在无损情况下探知叶片光合机构的损伤程度,快速叶绿素荧光测定和分析技术(JIP-test)将测量值转化为多种具有生物学意义的参数,因而被广泛应用于植物光合机构对环境的响应机制研究。该文研究了超大甜椒(Capsicum annuum)幼苗在强光及不同NaCl浓度胁迫下的荧光响应情况。与单纯强光胁迫相比,NaCl胁迫引起了叶绿素荧光诱导曲线的明显改变,光系统II(PSII)光抑制加重,同时PSII反应中心和受体侧受到明显影响,而且高NaCl浓度胁迫下PSII供体侧受伤害明显,同时PSI反应中心活性(P700+)在盐胁迫下明显降低。这些结果表明,NaCl胁迫会增强强光对超大甜椒光系统的光抑制,并且浓度越高抑制越明显,但对PSI的抑制作用低于PSII。高NaCl浓度胁迫易对PSII供体侧造成破坏,且PSI光抑制严重。
Rapid chlorophyll fluorescence kinetics can detect the extent of damage to leaf photosynthetic apparatus under non-destructive conditions. Rapid chlorophyll fluorescence assay and analysis technology (JIP-test) converts the measured values into a variety of biologically meaningful parameters and is therefore widely used in plants Response Mechanism of Photosynthetic Apparatus to Environment. In this paper, the fluorescence response of Capsicum annuum seedlings to light and NaCl stress was studied. Compared with pure light stress, NaCl stress induced a significant change of chlorophyll fluorescence induction curve, photoperiod II (PSII) photoinhibition aggravated, while PSII reaction center and receptor side were significantly affected, and under high NaCl concentration stress PSII Body damage was obvious, while PSI reaction center activity (P700 +) was significantly reduced under salt stress. These results indicate that NaCl stress enhances the photoinhibition of super light pepper light system under light stress, and the higher the concentration, the more obvious the inhibition, but the lower the inhibition effect on PSI is. High concentrations of NaCl stress easily damage the PSII donor side, and PSI light was severe.