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体外循环条件下（降温至３０℃）顺灌或逆灌冷钾稀释血（４℃）实施瓣膜置换术中，应用焦锑酸示踪法作为钙探剂对２０例风心病患者右心房肌线粒体及Ｃａ~（２＋）含量做了动态定量研究。结果表明，顺灌时在停跳开始（时相Ⅰ）线粒体结构变化极轻，至缺血４０分（时相Ⅱ）含钙线粒体及所含钙粒的Ｖｖ（体密度））增长不显著。逆灌组在同时相出现钙高峰，说明膜通透性升高，其钙高峰出现于时相Ⅱ，含钙线粒体及所含钙粒的体密度分别增大１３倍和５倍。这与顺灌钙高峰的水平相一致，但较顺灌提前一个时相发生，顺灌组在复跳期（时相Ⅲ）才出现钙高峰，含钙线粒体及所含钙粒的Ｖｖ剧增，较时相Ⅱ分别增加９４％和８２％，显示逆灌能使心肌组织获得更充分的灌注，顺灌组复跳时钙的剧增可能是瞬间血流再灌注导致的损伤。逆灌组第２个钙高锋出现于时相Ⅲ，其水平高于时相Ⅱ，但无统计学意义，到时相Ⅳ则急剧下降，而顺灌组的含钙线粒体及所含钙颗粒的体密度则基本未变。这说明逆灌的保护效果优于顺灌，复跳２０分时心肌细胞的结构和功能基本恢复到原始状态，能进一步降低受累心肌对缺血的致敏性（逆灌组耐缺血时间约为１００分钟，顺灌组约为８０分钟）。
Under the condition of cardiopulmonary bypass (cooling to 30 ° C), blood was diluted with cold potassium (4 ° C) to perform valvular replacement. The antimotica tracer method was used as a calcium probe to detect mitochondria in right atrial muscle of 20 patients with rheumatic heart disease And Ca ~ (2+) content were dynamically and quantitatively studied. The results showed that the mitochondrial structure changed very little at the beginning of arrest (phase Ⅰ) and no significant increase of Vv (body density) was observed in the calcium-containing mitochondria and calcium granules at 40 minutes after ischemia (phase Ⅱ). At the same time, the peak of calcium appeared at the same time, indicating that the membrane permeability increased and the calcium peak appeared in phase Ⅱ. The body density of calcium-containing mitochondria and calcium particles increased by 13 times and 5 times respectively. This coincides with the level of cis-acting calcium peak, but compared with cis-irrigation one phase in advance, cis-acting group in the rebound phase (phase Ⅲ) appeared calcium peak, calcium-containing mitochondria and calcium particles Vv increased dramatically , Respectively, compared with phase Ⅱ increased by 94% and 82%, respectively, indicating that reverse perfusion can make myocardial tissue more fully perfusion, smooth muscle reperfusion Shun group may be caused by transient blood flow reperfusion injury. The second calcium hyperflux in the anti-irrigation group appeared in phase Ⅲ, and its level was higher than that in phase Ⅱ, but it was not statistically significant, but decreased sharply in phase Ⅳ. However, the calcium-containing mitochondria and calcium particles The body density is basically unchanged. This shows that the protective effect of inverse irrigation is better than Shun irrigation, rebound 20 points when the basic structure and function of myocardial cells returned to its original state, can further reduce the affected myocardial ischemia on the allergenicity (anti-ischemic ischemia-resistant group of about For 100 minutes, Shun irrigation group about 80 minutes).