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目的 :探讨 L-精氨酸对肠缺血再灌注时大鼠肺及血清中 IL- 6、IL- 8及 TNFα的影响。方法 :制作幼鼠小肠缺血再灌注模型 ,用 L-精氨酸进行实验性预防 ,应用放射免疫法测定 TNFα含量 ,ELISA法测定 IL-6、IL- 8水平。结果 :S组 IL- 6水平显著高于 IR组及 L-精氨酸组 ( P<0 .0 1 ) ,IL- 8和 TNFα在各组中差异无显著性。结论 :L-精氨酸对肠缺血再灌注损伤早期血清及肺中炎症介质 IL- 6、8及 TNFα水平的影响不明显。
Objective: To investigate the effect of L-arginine on IL-6, IL-8 and TNFα in lung and serum of rats after intestinal ischemia / reperfusion. Methods: The intestinal ischemia-reperfusion model was made in neonatal rats. L-arginine was used for experimental prevention. The content of TNFα was determined by radioimmunoassay. The levels of IL-6 and IL-8 were determined by ELISA. Results: The level of IL-6 in S group was significantly higher than that in IR group and L-arginine group (P <0.01). There was no significant difference in IL-8 and TNFα between groups. Conclusion: L-arginine has no obvious effect on IL-6, 8 and TNFα levels in serum and lung in early stage of intestinal ischemia-reperfusion injury.