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用盐提法从新鲜向日葵花盘中得到果胶酯酶(PE)的粗提取液,用pH-stat法测得的PE酶活值较高。此种PE的最适温度为45℃左右。50℃以上PE的热稳定性下降,55℃下恒温20分钟,PE的活力下降60%.向日葵花盘中PE的活力受花盘的成熟度等因素影响。
The crude extract of pectin esterase (PE) was obtained from the fresh sunflower disk by salt extraction, and the PE activity measured by pH-stat method was higher. The optimum temperature for this kind of PE is about 45 ℃. The thermal stability of PE above 50 ℃ decreased, the temperature was maintained at 55 ℃ for 20 minutes and the viability of PE decreased by 60%. The vitality of PE in sunflower disk is affected by the maturity of the disk and other factors.