论文部分内容阅读
目的 建立一种可靠的小鼠脑缺血模型，了解永久性和暂时性阻断血管后的脑病理生理变化。方法 Ｃ Ｄ１ 小鼠，单侧大脑中动脉线栓阻断，监测脑血流，测量梗塞体积和血脑屏障破坏程度。结果 小鼠均出现成熟缺血核心区和半暗区。不同缺血／ 再灌注时间产生不同缺血损害和血脑屏障破坏，但有时限。结论 建立了一种可靠、复制性强的小鼠局灶性缺血模型。血脑屏障破坏在缺血损害中起重要作用。短时间缺血后的再灌注能减轻缺血性脑损害。
Objective To establish a reliable model of cerebral ischemia in mice and to understand the pathophysiological changes after permanent and transient occlusion of blood vessels. Method C D 1 mice, unilateral middle cerebral artery occlusion line plug, monitoring cerebral blood flow, measuring infarct size and extent of damage to the blood-brain barrier. Results Mice developed mature ischemic core area and semi-dark area. Different ischemia / reperfusion time produces different ischemic damage and blood-brain barrier damage, but there are time limits. Conclusion A reliable and reproducible mouse model of focal ischemia was established. Blood-brain barrier damage plays an important role in ischemic damage. Short-term ischemia reperfusion can reduce ischemic brain damage.